Chicory root fibres selectively increase bifidobacteria irrespective of food matrix

It has now been scientifically proven that prebiotic chicory root fibres’ benefit on Bifidobacteria growth is independent from the type of food it is used in

By Beneo

A recently published study (1) conducted by researchers from The Department of Food and Nutritional Sciences University of Reading (UK), in collaboration with the BENEO-Institute, demonstrates that prebiotic chicory root fibres support the selective growth of Bifidobacteria in the human gut, no matter which food application and food matrix they are used in. This is good news for producers wanting to bring the bifidogenic effect and gut health benefits of prebiotic chicory root fibre to a wide variety of consumer food products.

Encouraging the growth of beneficial bacteria such as Bifidobacteria is important as it supports human health via positive effects on the gut environment. It also creates less favourable living conditions for potential pathogen bacteria and has benefits beyond digestive health for overall well-being. The bioavailability and therefore, efficacy, of some ingredients and nutrients has been found to be impacted by the food matrix they are in. As a result, certain benefits cannot be credibly claimed by producers, as the ingredient’s function may differ depending on the application or matrix it is used in. However, the study shows that when incorporating prebiotic chicory root fibre into food application, this is not the case. This is a key factor for producers to consider given that three in four consumers who buy prebiotics state product efficacy as a key purchasing factor (76%) (2).

The study

Though several studies of inulin-type fructans in various food applications and matrices already exist, this is the first time that the effects of chicory root fibre in different food applications have been included within one study design, enabling their direct comparison. This study was designed as a prospective, parallel-group, randomised trial. The participants were split into four groups – with 24 participants in each. The foods reflected a wide range of matrices, such as baked, semi solid and liquid, and were consumed as part of the populations’ habitual diet. While the first group received pure inulin (for comparison purposes), the other participants were given inulin-enriched foods in the format of shortbread, milk chocolate, or a rice drink, depending on which group they belonged to. All groups consumed a total of 10g of chicory root fibre (BENEO’s Orafti® Inulin) per day (5g in the morning and 5g in the evening), and stool samples were taken at the beginning and at the end of the ten-day intervention.

At the end of the intervention period, the four groups’ results were analysed, and, in all cases, the bacterial enumeration demonstrated a significant increase in Bifidobacteria on day ten. In fact, an average 92% increase of Bifidobacteria was seen across all four groups compared to the baseline, and no significant differences were detected between any of the intervention groups on day ten. Irrespective of the food application and matrix, the prebiotic chicory root fibres were shown to support the selective growth of Bifidobacteria and the results were consistent across two different study methods used within the research. Thanks to these findings, product developers can have even more science-based trust in the functional benefits of BENEO’s chicory root fibres.

These findings add to the vast number of studies about the beneficial effects of chicory root fibres that already exist and are based on more than 25 years of scientific research on the proven prebiotics inulin and oligofructose.

BENEO’s chicory root fibres, Orafti® Inulin and Oligofructose, are the only proven plant-based prebiotics, comply with the ISAPP (International Scientific Association for Probiotics and Prebiotics) definition of prebiotics (3) and are backed up by the highest level of scientific evidence established in more than 25 years of scientific research. The prebiotic effect of chicory root fibres has been shown to support a number of health aspects in numerous human intervention studies, including improved bowel function, the inner defense system, weight and blood sugar management.


1. Jackson PPJ, Wijeyesekera A, Theis S, Van Harsselaar J, Rastall RA (2023) Effects of food matrix on the prebiotic efficacy of inulin-type fructans: a randomised trial. Beneficial microbes. Published 29 August 2023:

2. FMCG Gurus, Prebiotics survey, Q2 2022. A total of 10,000 consumers were surveyed across 10 countries (1,000 per country), here: filtered by consumers who purchase prebiotics, N = 1,260.

3. Gibson GR, Hutkins R, Sanders ME et al. (2017) Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nat Rev Gastroenterol Hepatol 14(8): 491–502.

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